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. 2012 May 9;7(5):e36457. doi: 10.1371/journal.pone.0036457

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Smal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) CD spectra of E7C (full line), E7C protein after 2 hours (broken and dotted line) and overnight incubation (broken line) with EDTA. (B) Size distribution measured by DLS. The distribution profiles correspond to: E7SOs (white bars), E7C incubated 2 hours with EDTA (grey bars) and E7C incubated 24 hours with EDTA (black bars). (C) Kinetic measurement of the oligomerization assembly after the addition of EDTA monitored by Thioflavin T fluorescence. E7C oligomerization kinetic (black full line) and E7SOs kinetic formation (grey full line). The time axis is in a log scale.