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. 2012 May 9;7(5):e36210. doi: 10.1371/journal.pone.0036210

Figure 1. Auxin promotes hypocotyl elongation in light-grown seedlings.

Figure 1

Auxin promotes hypocotyl elongation in a range of day-length conditions. Average hypocotyl length of wild-type seedlings grown in short days (SD; 8/16), long days (LD; 16/8) or constant light (LL) and treated with 5 µM picloram was determined following 24, 48, or 72 hours of auxin treatment. Hypocotyl length on auxin is shown as a percentage of length on control medium. Error bars indicate standard error. (B) Auxin response in seedlings increases with auxin concentration. Images of aerial portions of individual 7 day-old seedlings were captured following 48 hours of IAA treatment at the indicated concentrations. (C) Hypocotyl auxin response requires auxin signaling. Average hypocotyl length of wild-type or aux/iaa mutant seedlings treated with 5 µM picloram (red bars) or IAA (blue bars) was measured following 48 hours of auxin treatment. Hypocotyl length on auxin relative to the untreated control is shown as in (a). Error bars indicate standard error. Statistical significance was determined using a Tukey HSD post hoc comparison among the means on the analysis of variance using type III sums of squares (p<0.05).