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. Author manuscript; available in PMC: 2013 May 15.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 1;897:80–84. doi: 10.1016/j.jchromb.2012.03.035

Table 2.

LC/MS Matrix Effects, Recovery and Efficiency Testing by UPLC Overall Matrix Effects, Recovery and Efficiency

	LQC 1.50 ng/mL			MQC 17.5 ng/mL			HQC 35.0 ng/mL
	Neat	Post	Pre	Neat	Post	Pre	Neat	Post	Pre
n	3	3	3	3	3	3	3	3	3
Mean^*	0.125	0.118	0.113	1.42	1.55	1.48	2.95	3.06	3.09
SD	0.00506	0.00796	0.00925	0.0425	0.0430	0.106	0.146	0.0989	0.344
%CV	4.06	6.72	8.21	3.00	2.77	7.18	4.95	3.23	11.1

	Matrix Effect (post/neat)	Recovery Effect (pre/post)	Process Effect (pre/neat)
LQC (1.50 ng/mL)	94.9%	95.0%	90.2%
MQC (17.5 ng/mL)	109%	95.6%	104%
HQC (35.0 ng/mL)	104%	101%	105%

Mean	103%	97.2%	99.8%
SD	7.22	3.27	8.30
%CV	7.04	3.36	8.32

^*

Mean values = Area of DET-Me/IS response