Table 5.
Action of different antioxidants and MPT pore inhibitors on viability of PC12 cells assessed by LDH release after different time of incubation with different concentration of Cd2+.
| Treatment | Control cells (3, 5, 24 h) | 500 μM Cd (3 h) | 100 μM Cd (5 h) | 100 μM Cd (24 h) |
|---|---|---|---|---|
| Control | 100 | 55 ± 3* | 74 ± 4* | 61 ± 5* |
| Ruthenium Red (10 μM) | 99 ± 3 | 71 ± 6** | 92 ± 3** | 60 ± 4 |
| BKA, 5 μM | 100 ± 2 | 70 ± 5** | 82 ± 6 | 58 ± 2 |
| CsA, 1 μM | 101 ± 2 | 76 ± 4** | 72 ± 6 | 59 ± 18 |
| NAC (10 mM, 2 h) | 104 ± 4 | 94 ± 2** | 96 ± 2** | 92 ± 5** |
| Vit E, 500 μM | 103 ± 1 | 65 ± 6** | 86 ± 7** | 83 ± 2** |
| Mannitol (50 mM) | 100 ± 1 | 92 ± 6** | ||
| BHT, 150 μM | 99 ± 2 | 93 ± 1** | 82 ± 1** | |
| TEMPO (1 mM) | 97 ± 3 | 87 ± 8** |
The cell viability is expressed in % to untreated control. Mean ± SE; n = 3–11 independent experiments; *P < 0.05 compared to untreated control; **P < 0.05 compared to Cd(II)-treated control.