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. 2011 Oct 3;31(18):2335–2349. doi: 10.1038/onc.2011.415

Figure 5.

Figure 5

Low-risk HPV type-11 E6 downregulates CARM1 and PRMT1 activities in vivo. (a) 11E6 represses the CARM1- and PRMT1-induced GADD45 mRNA level and the corresponding histone modifications on the GADD45 promoter. U2OS cells ectopically expressing HA-CARM1 and HA-PRMT1 with or without 11E6 were subjected to RT–PCR, western blotting and ChIP assays. As shown, overexpression of CARM1 and PRMT1 increased the GADD45 mRNA level (compare lane 2 with lane 1) as well as the corresponding histone modifications on the GADD45 promoter (compare lane 5 with lane 4). In the presence of 11E6, all these CARM1- and PRMT1-stimulated effects were attenuated (compare lane 3 with lane 2 and lane 6 with lane 5), indicating that 11E6, which does not degrade p53 (as seen in the western blotting), could antagonize the CARM1- and PRMT1-mediated expression of the p53-target gene. (b) 11E6 downregulates the Adr-induced GADD45 mRNA level and the corresponding histone methylation and acetylation on the GADD45 promoter. U2OS cells with or without 11E6 and Adr were subjected to RT–PCR for GADD45 mRNA analysis and ChIP assays using Ab against Asy-H4R3me2 or Ace-H4.