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. 2011 Oct 3;31(18):2335–2349. doi: 10.1038/onc.2011.415

Figure 6.

Figure 6

E6 inhibits p53 binding to chromatin. H1299 cells were transfected with the indicated expression vectors and treated with MG132 followed by ChIP (a, top and middle panels), western (a, bottom panel) and DAPA (b) assays. (a) E6 suppresses p53 binding to endogenous p21 (upper panel) and GADD45 (middle panel) promoters. (b) E6 does not affect p53 binding to the non-chromatinized DNA containing the p53-binding site of the p21 promoter. (c) Knocking down E6 increases p53 binding to the p21 promoter. (d) Knocking down p53 reduces E6 binding to the p21 promoter. (c, d) HeLa cells were treated with MG132 or transfected with the indicated siRNAs, followed by ChIP, RT–PCR and western blot analysis.