Table 2.

Single locus segregation of fld 1 and fld 2 mutations, two aconitase (Aco1 & 2), and S-nitrosoglutathione reductase 2 (Gsnor 2) isozyme loci in F₂ and backcross (BC₁) populations of different intercrosses among four parents in Lathyrus sativus L. ^aFF-Homozygote of fast alleles, SS-Homozygote of slow allele, FS-Heterozygotes. *, **, and *** consistent with 1 : 2 : 1, 1 : 1, and 3 : 1 ratios, respectively, at 5% level of significance, ⁺⁺parent/s showing slow allozyme used in testcross with F₁ and pooled data of several crosses presented.

Cross++	Locus	Phenotype (F1)	F2/BC1 phenotypea			N	𝒳 2
			Normal		Deficient		(3 : 1/1 : 2 : 1/1 : 1)
Flavonoid mutant
BioL-212 × fldL-1	fld 1	Normal flavonoid	61	—	23	84	0.25***
F1 × fldL-1	fld 1	—	54	—	43	97	1.25**
HL × fldL-1	fld 1	Normal flavonoid	81	—	28	109	0.02***
F1 × fldL-1	fld 1	—	37	—	30	67	0.72**
BioL-212 × fldL-2	fld 2	Normal flavonoid	118	—	41	159	0.05***
F1 × fldL-2	fld 2	—	33	—	27	60	0.60**
HL × fldL-2	fld 2	Normal flavonoid	120	—	43	163	0.16***
F1 × fldL-2	fld 2	—	48	—	42	90	0.04**
			Normal	fldL-1	fldL-2	Double	𝒳 2
				type	type	recessive	(9 : 3 : 3 : 1)
fldL-1 × fldL-2	fld 1/fld 2	Normal flavonoid	153	50	57	20	0.96
Isozyme loci	Locus	Alleles	FF	FS	SS	N	𝒳 2
							(3:1/1:2:1/1:1)
BioL-212/HL × fldL-2	Gsnor 2	ab	47	101	51	199	0.20*
F1 × fldL-2	Gsnor 2	ab	—	54	61	115	0.43**
fldL-1 × fldL-2	Gsnor 2	ab	60	118	54	232	0.36*
F1 × fldL-2	Gsnor 2	ab	—	50	46	96	0.16**
fldL-1/fldL-2
× BioL-212/HL	Aco 1	ab	25	54	25	104	0.15*
F1 × BioL-212/HL	Aco 1	ab	—	37	44	81	0.60**
fldL-1 × fldL-2	Aco 2	ab	51	92	44	187	0.57*
F1 × fldL-2	Aco 2	ab	—	44	38	82	0.44**
BioL-212/HL
× fldL-2	Aco 2	bc	46	88	39	173	0.62*
F1 × BioL-212/HL	Aco 2	bc	—	40	34	74	0.49**
BioL-212/HL × fldL-1	Aco 2	ac	23	38	20	81	0.53*
F1 × BioL-212/HL	Aco 2	ac	—	17	13	30	0.53**