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. 2011 Nov 4;3(1):31–39. doi: 10.1021/cn200082q

Figure 3.

Figure 3

3D environment promotes further differentiation of MLPCs. (A–F) Phase contrast images of differentiating MLPCs. (A) The untreated cells displayed typical fibroblast morphology. (B) Cells exhibited flattening and spreading at 24 h in the 3D environment. (C) Cells after 8 days in the differentiation medium displayed an increased level of flattening. (D–F) After differentiation for 30 days, approximately 80% of cells revealed extensive processes. (G–I) Growth factors influenced the development of processes. (G and H) Immunostained cells displaying increased levels of branching and development of processes in the presence of bFGF and EGF. (I) Simple processes were observed in the absence of bFGF and EGF. Scale bars are 100 μm (40× magnification).