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. 2012 Apr 17;106(10):1660–1667. doi: 10.1038/bjc.2012.139

Figure 3.

Figure 3

4EGI-1 induced Bax activation, and simultaneous knockdown of Bak and Bax inhibited 4EGI-1-induced apoptosis. (A) Cell lines were treated with 50 μℳ 4EGI-1 for 18 h in culture conditions. Cells were then quantified for Bax activation using the conformation-dependent anti-Bax antibody (6A7). (B) LP-1 cells were transfected with the indicated siRNA for 72 h before being treated with 50 μℳ 4EGI-1 for 18 h. Equivalent amounts of cell lysates were separated by SDS–PAGE and then immunoblotted with the indicated antibodies. Actin was used as a loading control. (C) Cell death was assessed by Annexin V staining in LP1 cells.