Figure 4.

Effects of serum derived from ticlopidine- or aspirin-treated rats on HUVEC proliferation (A) and apoptosis (B). HUVECs were incubated with sera from vehicle-treated, ticlopidine-treated (300 mg/kg), or aspirin-treated (30 mg/kg) rats for 24 h. Cell proliferation was determined by the MTT assay, and apoptosis was assessed by DAPI staining (B). *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. the corresponding vehicle-treated group. The dotted lines show the mean levels of proliferation or apoptosis in cells incubated only with the control medium (containing 2.5% FBS, but no rat serum).