Table 3.
The Box-Behnken design of RSM for optimization of protease production.
| Variable | KH2PO4 | pH | Temperature | Protease activity |
|---|---|---|---|---|
| Variable code | X1 | X2 | X3 | |
| Run | Units | |||
| (g/L) | (°C) | (U/mL) | ||
|
| ||||
| (1) | 1 | 5 | 27.5 | 58.11 |
| (2) | 1 | 6.5 | 25 | 44.11 |
| (3) | 1 | 6.5 | 30 | 82.14 |
| (4) | 1 | 8 | 27.5 | 57.09 |
| (5) | 1.5 | 8 | 25 | 39.64 |
| (6) | 1.5 | 5 | 25 | 37.49 |
| (7) | 1.5 | 5 | 30 | 67.17 |
| (8) | 1.5 | 8 | 30 | 64.19 |
| (9) | 2 | 5 | 27.5 | 56.82 |
| (10) | 2 | 8 | 27.5 | 53.17 |
| (11) | 2 | 6.5 | 25 | 47.72 |
| (12) | 2 | 6.5 | 30 | 53.09 |
| (13) | 1.5 | 6.5 | 27.5 | 56.5 |
| (14) | 1.5 | 6.5 | 27.5 | 57.72 |
| (15) | 1.5 | 6.5 | 27.5 | 52.66 |
| (16) | 1.5 | 6.5 | 27.5 | 54.47 |
| (17) | 1.5 | 6.5 | 27.5 | 56.39 |