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Effect of some metal ions and inhibitors on A. niger I1 protease activity.
| Chemicals | Concentration (mM) | Relative activity (%) |
|---|---|---|
| Control | — | 100 |
| Ca2+ | 5 | 100 ± 2 |
| Mg2+ | 5 | 86 |
| Mn2+ | 5 | 85 ± 3 |
| Cu2+ | 5 | 61 ± 4 |
| Ba2+ | 5 | 87 |
| Hg2+ | 5 | 78 ± 2 |
| Na+ | 5 | 93 ± 2 |
| K+ | 5 | 110 ± 3 |
| Zn2+ | 5 | 97 |
| PMSF | 5 | 100 |
| EDTA | 5 | 100 |
| DTNB | 5 | 100 |
| Pepstatin A | 1.5 × 10−3 | 0 |
| β-mercaptoethanol | 5 | 100 ± 3 |
