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. Author manuscript; available in PMC: 2012 Sep 29.
Published in final edited form as: Nature. 2012 Mar 28;483(7391):570–575. doi: 10.1038/nature11005

Figure 4. Ewing’s sarcoma cell lines are sensitive to PARP inhibition.

Figure 4

a, The IC50 values of WT and EWS-FLI1 fusion positive cell lines to olaparib and AG-014699. b, Dose response curves to olaparib following 6-days constant drug exposure. Cell lines are classified according to tissue sub-type. c, Colony formation assays were performed for 7-21 days over a range of olaparib concentrations (0.1, 0.32, 1, 3.2 or 10 uM) and the concentration at which the number of colonies is reduced >90% for each cell line is indicated. d, Olaparib induced apoptosis in Ewing’s sarcoma cell lines following 72 hours treatment. e, Sensitivity to olaparib of EWS-FLI1 and FUS-CHOP transformed mouse mesenchymal cells compared to the SK-N-MC cell line (which harbors the EWS-FLI1 fusion). f, Sensitivity to olaparib of A673 cells transiently transfected with (siEF1) and without (siCT) EWS-FLI1 specific siRNA. All error bars are s.d from triplicate measurements except for b where error bars have been removed for clarity.