Table 1.

Normal and X and Y Disomy Frequencies in Sperm Nuclei Using Manual Versus Semi-Automated Scoring Methods of Triple-Probe FISH.

			X18			Y18			XX18			YY18			XY18			Total Disomy
Slide	Meth	N	n	%	P	n	%	P	n	%	P	n	%	P	n	%	P	n	%	P
1	manual	643	290	45.10	0.86	133	20.68	0.83	1	0.16	1	1	0.16	0.62	1	0.46	1	3	0.47	0.34
	auto	443	294	45.72		137	21.31		2	0.31		3	0.47		2	0.31		7	1.09
2	manual	499	196	39.28	0.90	160	32.06	0.68	1	0.20	1	2	0.40	0.68	0	0.00	1	3	0.60	0.73
	auto	499	199	39.88		153	30.66		1	0.20		4	0.80		0	0.00		5	1.00
3	manual	427	202	47.31	1.00	174	40.75	1.00	1	0.23	1	1	0.23	1	0	0.00	1	2	0.47	1
	auto	427	202	47.31		173	40.52		0	0.00		1	0.23		0	0.00		1	0.23
4	manual	411	179	43.55	1.00	161	39.17	0.94	3	0.73	0.62	1	0.24	1	1	0.24	1	5	1.22	1
	auto	411	179	43.55		159	38.69		1	0.24		2	0.49		1	0.24		4	0.97
5	manual	532	178	33.46	0.80	159	29.89	1.00	0	0.00	0.25	4	0.75	0.75	3	0.56	1	7	1.32	0.35
	auto	532	183	34.40		160	30.08		3	0.56		6	1.13		3	0.56		12	2.26
6	manual	517	138	26.69	0.57	129	24.95	0.42	2	0.00	0.50	0	0.00	0.12	4	0.77	0.17	4	0.77	0.01
	auto	517	129	24.95		117	22.63		0	0.39		4	0.77		10	1.93		16	3.09
7	manual	1024	352	34.38	1.00	340	33.20	0.67	0	0.00	1	1	0.10	1	13	1.27	0.58	14	1.37	0.48
	auto	1024	352	34.38		330	32.23		0	0.00		2	0.20		17	1.66		19	1.86
8	manual	620	263	42.42	1.00	276	44.52	0.68	0	0.00	1	0	0.00	0.06	4	0.65	1	4	0.65	0.26
	auto	620	263	42.42		268	43.23		0	0.00		5	0.81		1	0.65		9	1.45
9	manual	330	101	30.61	0.001	99	30.00	0.04	3	0.91	0.09	1	0.30	0.12	0	0.00	0.50	4	1.21	0.01
	auto	330	58	17.58		75	22.73		10	3.03		6	1.82		2	0.61		18	5.45
10	manual	501	231	46.11	0.89	164	32.73	0.63	0	0.00	0.25	0	0.00	0.50	2	0.40	0.28	2	0.40	0.02
	auto	501	228	45.51		172	34.33		3	0.60		2	0.40		6	1.20		11	2.20

Meth = automated or manual scoring; P= p value for comparison of each slide using Pearson's chi-square or Fisher's exact tests