Table 1.
Phylogenetic affiliations of archaeal phylotypes based on 16S rRNA gene sequences in the produced water from a CBM well in the PRB.
| Sequence ID | No of Sequences | Closest cultured relative (% identity) | Maximum identity to GenBank (% identity) | Environmental source |
|---|---|---|---|---|
| OTU-1 | 22 | Methanobacterium sp. GH, [EU333914], (99) | Uncultured archaeon clone DR9IPCA16SCT2, [AY604061], (99) | Deep dolomite aquifer, South Africa |
| OTU-10 | 3 | Methanobacterium sp. GH, [EU333914], (96) | Uncultured archaeon clone DR9IPCA16SCT2, [AY604061], (96) | Deep dolomite aquifer, South Africa |
| OTU-7 | 15 | Methanobacterium sp. SA-12, [AY695843], (97) | Uncultured archaeon clone MH1100_B4C [EU155989], (97) | Rich minerotrophic fen, New York State, USA |
| OTU-8 | 5 | Uncultured archaeon clone:pMLA-5 [AB109882], (93) | Gas hydrate production research well, Canada | |
| OTU-6 | 2 | Uncultured archaeon clone MD3059N-45 [GQ927542], (92) | Deep aquifer from gold mines, South African | |
| OTU-45 | 2 | Uncultured archaeon SAGMA-E [AB050209], (97) | Deep aquifer from gold mines, South African | |
| OTU-16 | 8 | Uncultured archaeon clone MD3059S-72 [GQ927695], (96) | Deep-sea sediments from tropical western Pacific | |
| OTU-30 | 2 | Uncultured archaeon clone 4H3_ar33 [DQ302026], (96) | Pacific deep subsurface sediments | |
| OTU-38 | 8 | Uncultured archaeon clone MD3059P-24 16, [GQ927572], (91) | Hydrothermal sediments, Japan |