Figure 1.

Schematic of conditional il2 gene deletion. (A) Step 1: il2 gene knock-out constructs schematic shows floxed targeted region including exons I and II (1402 bp) and Neo cassette inserted between exons II and III. The primers DL1, Lox1, UNI, LAN1 and A3, and FRT and loxP sites are indicated. Step 2: il2 gene schematic after Neo cassette deletion upon FLP mating and the primers DL1, Lox1, SG1, and A3 is also indicated. Step 3: Schematic of il2 gene of TAM-treated Cre+ (Cre-ERT2/flox-il2) mice is shown. (B) Schematics of il2 gene in the Cre− mice before TAM treatment and the Cre+ mice upon deletion of exons I and II by TAM.