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The efficiency of TAM-induced il2 deletion. The twofold dilution of [IL-2] (pM) by ELISA assay in WT (●; n = 11), conventional IL-2KO (◼; n = 13), conditional IL-2(−/−) Cre+ (▴; n = 13), and Cre− (▾; n = 9) mice (p < 0.0001). Anti-CD3/28-activated splenocytes were cultured in vitro for 20–24 h. The results are shown as Mean ± SEM (vertical brackets).
