Table 1.
Cytokine production.
| Exp# | IL-2 | IL-4 | IL-7 | IL-9 | IL-13 | IL-15 | IL-21 | IL-1β | IL-6 | IL-12 | TNF-α | IL-17 | IFN-γ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | |||||||||||||
| 1 | 3321.4 | 85.3 | 0.7 | 0.0 | 190.5 | 0.0 | 12.2 | 23.3 | 2245.6 | 0.0 | 188.8 | 1149.7 | 14364.0 |
| 2 | 2227.6 | 32.0 | 0.0 | 0.0 | 191.7 | 0.0 | 63.0 | 187.0 | 5778.5 | 53.1 | 310.3 | 4041.3 | 12957.1 |
| 3 | 333.7 | 148.5 | 0.0 | 50.8 | 287.7 | 0.0 | 72.5 | 68.8 | 5188.3 | 0.0 | 549.8 | 2325.3 | 16376.3 |
| 4 | 453.6 | 120.6 | 0.0 | 37.7 | 209.0 | 0.1 | 23.0 | 58.3 | 4804.4 | 0.0 | 380.2 | 840.8 | 14299.8 |
| 5 | 1147.5 | 158.5 | 0.0 | 63.4 | 254.3 | 0.4 | 14.4 | 51.1 | 4597.7 | 0.0 | 680.7 | 2456.4 | 17158.5 |
| Mean | 1496.8 | 109.0 | 0.1 | 30.4 | 226.6 | 0.1 | 37.0 | 77.7 | 4522.9 | 10.6 | 421.9 | 2162.7 | 15031.2 |
| SEM | 566.8 | 23.1 | 0.1 | 13.0 | 19.1 | 0.1 | 12.8 | 28.3 | 603.7 | 10.6 | 87.1 | 566.4 | 762.2 |
| IL-2KO | |||||||||||||
| 1 | 0.0 | 7.1 | 1.6 | 0.0 | 0.0 | 4.6 | 1.5 | 210.3 | 4093.9 | 0.0 | 178.0 | 470.5 | 437.0 |
| 2 | 2.1 | 2.0 | 0.2 | 0.0 | 40 | 0.6 | 25.8 | 90.3 | 1841.5 | 0.0 | 163.9 | 881.2 | 42.3 |
| 3 | 0.0 | 1.9 | 0.0 | 9.3 | 9.6 | 0.0 | 11.1 | 139.4 | 14887.3 | 0.0 | 638.2 | 2040.4 | 871.7 |
| 4 | 2.0 | 10.8 | 0.0 | 26.6 | 3.2 | 0.6 | 0.0 | 44.1 | 1407.0 | 0.0 | 420.7 | 234.3 | 274.0 |
| 5 | 0.0 | 0.8 | 0.0 | 0.0 | 9.6 | 0.0 | 59.3 | 84.2 | 14594.3 | 0.0 | 394.5 | 3614.8 | 520.8 |
| Mean | 0.8 | 4.5 | 0.3 | 7.2 | 5.3 | 1.2 | 19.5 | 113.7 | 7364.8 | 0.0 | 359.1 | 1448.2 | 429.1 |
| SEM | 0.5 | 1.9 | 0.3 | 5.2 | 1.9 | 0.9 | 10.9 | 28.5 | 3054.9 | 0.0 | 87.7 | 624.3 | 137.5 |
| CRE− | |||||||||||||
| 1 | 1855.3 | 118.2 | 0.0 | 0.0 | 49.5 | 0.0 | 59.6 | 35.9 | 4542.7 | 23 | 701.3 | 350.2 | 14281.5 |
| 2 | 546.8 | 82.5 | 0.3 | 0.0 | 248.5 | 0.4 | 61.5 | 127.2 | 3410.1 | 0.0 | 265.2 | 1371.9 | 12913.8 |
| 3 | 914.2 | 84.8 | 0.0 | 19.4 | 71.0 | 0.0 | 14.0 | 60.3 | 5423.8 | 0.0 | 400.9 | 848.4 | 11976.4 |
| 4 | 653.4 | 150.5 | 0.0 | 66.2 | 140.5 | 0.7 | 113.4 | 80.1 | 6379.1 | 0.0 | 462.2 | 1107.5 | 12199.7 |
| 5 | 1244.8 | 120.2 | 0.0 | 35.0 | 117.7 | 0.2 | 149.3 | 67.2 | 5557.7 | 0.0 | 470.3 | 1508.8 | 11694.5 |
| Mean | 1042.9 | 111.2 | 0.1 | 24.1 | 125.6 | 0.3 | 79.6 | 74.2 | 5062.7 | 0.5 | 460.0 | 1037.4 | 12613.3 |
| SEM | 236.1 | 12.6 | 0.1 | 12.4 | 34.8 | 0.1 | 23.5 | 15.1 | 505.5 | 0.5 | 70.6 | 205.8 | 463.4 |
| CRE+ | |||||||||||||
| 1 | 24.2 | 143.6 | 0.0 | 0.0 | 66.9 | 1.6 | 444.2 | 48.4 | 5591.4 | 30.7 | 1095.8 | 930.9 | 14587.5 |
| 2 | 0.0 | 25.3 | 0.0 | 0.0 | 63.2 | 0.0 | 55.3 | 103.2 | 3771.8 | 14.6 | 250.2 | 791.0 | 12943.1 |
| 3 | 11.9 | 54.3 | 0.0 | 20.0 | 27.9 | 0.0 | 43.7 | 36.0 | 2784.8 | 0.0 | 290.3 | 268.3 | 11559.1 |
| 4 | 16.3 | 54.8 | 0.0 | 44.0 | 31.8 | 0.6 | 64.2 | 44.9 | 2857.3 | 0.0 | 410.4 | 187.2 | 13345.2 |
| 5 | 15.5 | 44.5 | 0.0 | 32.9 | 12.3 | 0.5 | 0.0 | 47.0 | 3830.8 | 0.0 | 326.1 | 166.8 | 11371.1 |
| Mean | 13.6 | 64.6 | 0.0 | 19.4 | 40.4 | 0.5 | 121.5 | 55.9 | 3767.6 | 9.1 | 474.6 | 468.8 | 12761.2 |
| SEM | 3.9 | 20.5 | 0.0 | 8.8 | 10.6 | 0.3 | 81.4 | 12.0 | 506.6 | 6.1 | 157.5 | 162.5 | 595.3 |
The concentrations (pg/mL) of 13 cytokines from WT, conventional IL-2(−/−), Cre−, and conditional IL-2(−/−) Cre+ mice. Protein-bead array was performed on supernatants with or without anti-CD3/28-activation for 24 h. The difference between anti-CD3/28-activation and the media of the control is shown, as the values for the non-stimulated controls were very low by comparison. The results from five individual experiments and the Mean ± SEM are shown.