Table 3.
Innate immunity-related transcripts from 454 sequencing of E. scolopes hemocytes.
| Putative ID | Accession no. | Organism | e-Value | Putative function | No. of unique peptides mappeda |
|---|---|---|---|---|---|
| NF-κB CASCADE | |||||
| TRAF6 | AAY27978.1 | E. scolopes | 4e-48 | NF-κB signaling | N/A |
| TRAF4 | ABN04153.1 | B. belcheri | 9e-50 | NF-κB signaling | N/A |
| REL/NF-κB | AAY27981.1 | E. scolopes | 9e-65 | NF-κB signaling | N/A |
| Inhibitor of NF-κB | AAY27980.1 | E. scolopes | 4e-4 | NF-κB signaling | N/A |
| NF-κB activating protein | CAX69433.1 | S. japonicum | 1e-36 | NF-κB signaling | N/A |
| Calcineurin subunit A | ABO26624.1 | H. discus | 5e-54 | Suppressor of NF-κB | N/A |
| Calcineurin subunit B | ACI96107.1 | P. fucata | 2e-56 | Suppressor of NF-κB | N/A |
| Regulator of calcineurin | NP_001011215.1 | X. tropicalis | 4e-11 | Regulator of calcineurin | N/A |
| MyD88 | BAC76897.1 | C. porcellus | 1e-20 | Toll signaling protein | N/A |
| MAMP BINDING | |||||
| Galectin | ACS72241.1 | A. irradians | 3e-43 | Glycan binding | 3 |
| PGRP-SC2 precursor (EsPGRP5) | ACV67267.1 | B. manjavacas | 5e-38 | PGN binding/cleavage | 4 |
| PGRP4 | AAY27976.1 | E. scolopes | 1e-52 | PGN binding | 4b |
| OTHER | |||||
| IK cytokine | ABV24915.1 | C. gigas | 7e-47 | Anti-interferon cytokine | N/A |
| CD63 antigen | ABY87409.1 | H. diversicolor | 3e-25 | Phagosome protein | 3 |
| Plancitoxin | Q75WF2.1 | A. planci | 1e-68 | DNA-degrading protein | 2 |
| MMP/vitronectin | BAC66058.1 | S. kowalevskii | 7e-7 | Immune homeostasis | 1 |
| Cephalotoxin | B2DCR8.1 | S. esculenta | 2e-118 | Toxin | 2 |
E. scolopes, Euprymna scolopes; B. belcheri, Branchiostoma belcheri; S. japonicum, Schistosoma japonicum; H. discus, Haliotis discus; P. fucata, Pinctada fucata; X. tropicalis, Xenopus tropicalis; C. porcellus, Cavia porcellus; A. irridians, Argopecten irradians; B. manjavacas, Brachionus manjavacas; C. gigas, Crassostrea gigas; H. diversicolor, Haliotis diversicolor; A. planci, Acanthaster planci; S. kowalevskii, Saccoglossus kowalevskii; S. esculenta, Sepia esculenta; PGN, peptidoglycan; MMP, Matrix metalloproteinase.
aMascot and Scaffold validated peptide matches using translated hemocyte ESTs (Dataset S2 in Supplementary Material).
bPGRP4 peptide matches identified in an additional search using light organ and hemocyte translated transcripts, which included published full-length E. scolopes protein sequences (Dataset S2 in Supplementary Material).