Table 5.
Putative complement pathway and other innate immunity-related proteins identified in E. scolopes hemocytes.
| Protein | Mascot scorea | Scaffold probabilityb | Unique peptidesc | Total spectra | E. scolopes protein sequence annotation using the NCBI nr database: organism|gi | e-Value |
|---|---|---|---|---|---|---|
| Complement component C3 | 24 | 60.20% | 1 | 1 | Euprymna scolopes|192383355 | 0 |
| Cephalotoxin | 39 | 99.80% | 1 | 1 | Sepia esculenta|221271983 | 2e-118 |
| Thioester protein (TEP) 1 | 218 | 99.80% | 2 | 5 | Chlamys farreri|144952812 | 2e-11 |
| TEP2 | 40 | 98.90% | 1 | 1 | Glossina morsitans morsitans|85822201 | 1e-13 |
| Alpha macroglobulin | 83 | 99.80% | 2 | 4 | Chlamys farreri|40074252 | 4e-7 |
| Matrix metalloproteinase/vitronectin | 45 | 99.80% | 2 | 2 | Saccoglossus kowalevskii|291237328 | 7e-7 |
| Sushi repeat domain-containing protein | 403 | 100.00% | 9 | 17 | Xenopus tropicalis|301626998 | 5e-31 |
| Hypothetical protein (similar to C1qBP) | 25 | Not validated | 1 | 1 | Branchiostoma floridae|260799758 | 6e-37 |
aProtein scores were derived from peptide ion scores and were determined by Mascot. Peptide scores greater than 20 were considered significant (p < 0.05).
bMascot identifications were validated by Scaffold, which utilizes Mascot scores to calculate protein identification probability.
cUnique peptides matched to protein sequences generated from light organ and hemocyte ESTs and published E. scolopes protein sequences present in the NCBI nr database (Dataset S2 in Supplementary Material).