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Effects of irinotecan and panaxadiol on HCT-116 cell apoptosis. HCT-116 cells were treated with panaxadiol (10 μM) and/or IRN (1, 3, 5, 10 or 20 μM) for 48 h. Apoptosis was quantified using flow cytometry after staining with annexin V/PI. (a) Representative scatter plots of PI (y-axis) vs annexin V (x-axis). (b) Percentage of viable, early apoptotic and late apoptotic cells. Data are presented as the mean ± SE of triplicate experiments.
