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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Stem Cells. 2012 Feb;30(2):140–149. doi: 10.1002/stem.778

Figure 5.

Figure 5

AICAR treatment down-regulates stability of Nanog protein. (A) R1 mES cells were preincubated with or without AICAR (0.5 mM) for 6h, and then cycloheximide (CHX, 50mg/ml) for indicated hours. Lysates were analyzed by immunoblotting for Nanog and β-tubulin (loading control). (B) Proteosome inhibitor (MG132) protects Nanog from AICAR treatment. After 6h culture with or without AICAR (0.5 mM), cells were treated with MG132 (30μM) or vehicle for 3h. Cells were harvested and lysed, and then Nanog and β-tubulin levels were analyzed. A representative blot of at three different experiments is shown. A diagram shows the experimental scheme.