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. 2011 Oct 28;302(2):L216–L225. doi: 10.1152/ajplung.00055.2011

Fig. 2.

Fig. 2.

Isoforms of CEACAM6 in lung fluid. A: representative Western for three d14 infants. Supernatant (Sup) contains bands at ∼90, 60, and 50 kDa, while surfactant pellets (Pellet) contain primarily 90-kDa CEACAM6. Recombinant CEACAM6 (rC6) is not fully glycosylated and runs at ∼55 kDa. Total protein loaded was 5 μg for supernatant, 1 μg for pellet, and 0.02 μg for rCEACAM6; different protein loading was required to provide comparable signals for scanning densitometry. B: densitometric quantitation of supernatant CEACAM6 distribution between isoforms at d1 and d14. C: densitometric quantitation of pellet CEACAM6 distribution between isoforms. The 90-kDa form was 65–80% of the total CEACAM6 in each comparison, except for supernatant d14 samples (30%). Values are means ± SE for 6 samples at d1 and 9 samples at d14. *P < 0.001 vs. d1.