Fig. 7.

Effect of culture media from CEES-exposed 16HBE cells on FX cleavage. 16HBE cultures were exposed either to no treatment (NT), diluent (DMSO) alone, or diluent containing CEES (750 μM). After 6 h, small volumes of media were obtained from these cultures and further incubated in buffer containing FX only (A) or buffer containing FX and factor VII (FVII) (B). After incubation (45 min), the reaction was stopped by adding sample loading buffer, and FX-to-FXa conversion was determined by Western blot. Blots shown were representative of 4 independent experiments.