Fig. 1.

Hyperoxia decreased Trek-1 expression in MLE-12 cells. Treatment of cells with 90% hyperoxia for 4 h (A) showed a 2.2-fold decrease in Trek-1 expression but did not significantly affect expression of the other 2-pore-domain K⁺ channels Trek-2 and TRAAK, or expression of the Ca²⁺-permeable channel transient receptor potential vanilloid (TRPV)-4. Exposure of cells to 16 h of hyperoxia (B) further decreased Trek-1 gene expression but remained without effect on Trek-2, TRAAK, and TRPV-4. A change in gene expression >2-fold was considered significant (*P < 0.05, n = 3 or greater).