Fig. 2.

Hyperoxia decreased Trek-1 protein expression in MLE-12 cells. A: representative time course for Trek-1 expression using Western blotting. No change in Trek-1 expression was observed after exposure of cells to hyperoxia for 24 h, but after 48 h Trek-1 expression was decreased. Densitometry analysis shows a summary of 7 independent measurements. Trek-1 expression was first normalized to GAPDH expression, and treatment groups were then normalized to control expression at 24 h. *P < 0.05. B: immunostaining for Trek-1 (green) in MLE-12 cells by confocal microscopy (magnification ×100). Exposure of cells to hyperoxia for 48 h decreased Trek-1 immunoreactivity. Nuclei were counterstained with DRAQ5 (blue). Representative images from 3 or more fields from 3 different experiments are shown.