Table 3.
Maximum response (Emax) and sensitivity (logEC50) to ACh or SNP in aortas from Dahl S rats at 16 wk old
| Teklad | Teklad→AIN | AIN | AIN→Teklad | |
|---|---|---|---|---|
| Emax to [ACh, 10−4.5 M] or [SNP, 10−5.5 M] | ||||
| ACh, %PE | 70.84 ± 4.18 (4) | 73.42 ± 5.62 (7) | 80.04 ± 2.63 (4) | 78.37 ± 1.30 (5) |
| ACh + l-NAME, %PE | −19.03 ± 4.01* (6) | −29.33 ± 8.13* (10) | −12.94 ± 4.38* (7) | −16.62 ± 7.27* (6) |
| SNP, %PE | 98.76 ± 0.68 (6) | 96.25 ± 1.6 (10) | 95.47 ± 2.53 | 99.39 ± 0.74 (6) |
| logEC50 | ||||
| ACh, M concn. | −6.31 ± 0.23 (4) | −6.33 ± 0.25 (7) | −6.49 ± 0.13 (4) | −6.49 ± 0.09 (5) |
| SNP, M concn. | −8.08 ± 0.11 (6) | −7.93 ± 0.15 (10) | −8.37 ± 0.13 (7) | −8.24 ± 0.13 (6) |
Values are means ± SE; no. of rats are in parentheses. Dahl S rats were given Teklad or AIN standard chow diets at weaning (3 wk old). At 12 wk, weaning-diet groups were divided, and a subset of rats underwent a diet switch, generating two additional diet groups: Teklad→AIN and AIN→Teklad. l-NAME was used at 100-μM concentration to nonselectively inhibit NOS.
*
P < 0.05 vs. corresponding untreated aortic artery segment. Data were analyzed by two-way ANOVA.