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. 2001 May 15;98(11):6482–6487. doi: 10.1073/pnas.111447798

Figure 4.

Figure 4

Bupivacaine inhibits ethanol-induced GIRK currents in absence of functional PTX-sensitive G proteins. (a) Oocytes were injected with the cRNAs for GIRK1, GIRK4, and m2 muscarinic receptor alone or with the cRNA for PTX-S1. Continuous current recordings show the response to 100 mM ethanol in the absence and then presence of 500 μM bupivacaine (VH = −80 mV). Note that bupivacaine also inhibited the PTX-resistant basal current. (b) Bar graph shows the average carbachol-induced currents in oocytes with or without coexpressed PTX-S1 (n = 5). (c) Bar graph shows the effect of 500 μM bupivacaine on ethanol-induced currents in the absence or presence of coexpressed PTX-S1 (n = 5). Asterisk indicates statistically significant difference (P < 0.05) between ethanol-induced current in the absence (c) and presence (b) of bupivacaine, using unpaired t test.