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. 2001 May 15;98(11):6482–6487. doi: 10.1073/pnas.111447798

Figure 5.

Figure 5

G protein-insensitive inwardly rectifying K+ channels are resistant to bupivacaine inhibition. Oocytes were injected with the cRNA for ROMK1, IRK1, R1G2(118–193), R1G2(97–168), or I1G2(96–189). (a–d) Current responses were elicited by voltage steps to +50 and −100 mV (VH = 0 mV) in the absence and then presence of extracellular bupivacaine (500 μM, pH 7.5). (e) Fractional current remaining (I/Io) shown for IRK1 (n = 4), ROMK1 (n = 5), I1G2(96–189) (n = 5), R1G2(118–193) (n = 5), and R1G2(97–168) (n = 5). (f) Western blot stained with anti-Gβ G protein antibody. Pull-down assay was used to measure the Gβ1γ7 binding to a glutathione S-transferase fusion protein containing the N- or C-terminal domain of GIRK1 in the absence (c) or presence (b) of 500 μM bupivacaine.