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Breeding strategy to generate double mutant mice. (A) Strategy to generate A53T-α-Syn mice on an LRRK2 null background. F0, F1 and F2 represent each generation of crossbreeding. The expected frequency of each genotype is indicated as a percent. (B) Strategy to generate bigenic mice co-expressing A53T-α-Syn and G2019S-LRRK2.
