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Immunoblot detection of AtHKT1-PhoA fusion proteins expressed in the E. coli UT5600 membrane. Membrane proteins were subjected to SDS/10% PAGE followed by Western blot analysis. D55-PhoA and S78-PhoA, 0.1 μg; K270-PhoA, 5 μg; the remaining PhoA fusions, 1 μg.
