Figure 7.

Immunocytochemical detection of the orientation of the N and C termini and three loops of AtHKT1 recombinantly expressed in HEK293 cells. Cells expressing AtHKT1 constructs tagged with Flag at the N terminus (A), the first external loop (D55-S62; B), the first internal loop (D135-Y142; C), the third internal loop (K377-S384; D), and the C terminus (E) were tested for accessibility to the Flag epitope of Flag-specific antibody in the presence (Triton +) and absence (Triton −) of Triton X-100. (Left) The representative confocal immunoimages; (Right) the corresponding differential interference contrast images. (Bar = 20 μm.)