Table 1.
Degenerate primer sequences used for viral screening and primer sets used for SDDM06-11
| Primer | Position a | Sequence, 5'-3' | Polarity |
|---|---|---|---|
| CxFV-SD-1-F | 21-39 | TGGTTACACCGCAGATTG | Sense |
| CxFV-SD-1-R | 1198-1217 | GATTGTAGGGCTGGGTTGAG | Reverse |
| CxFV-SD-2-F | 1034-1049 | AACGGACTTCTTGAGTTTCGC | Sense |
| CxFV-SD-2-R | 2197-2217 | GCCTTGGTGTAGACAAAGTATC | Reverse |
| CxFV-SD-3-F | 2002-2020 | GCAAGGTTGGAGAATGGC | Sense |
| CxFV-SD-3-R | 3238-3256 | GACCTTGAAGTGAAATACCC | Reverse |
| CxFV-SD-4-F | 3034-3055 | GTCTAAAGCAAACCACATTCC | Sense |
| CxFV-SD-4-R | 4233-4255 | CGGTCCGTAAGTTCCTTCTAAT | Reverse |
| CxFV-SD-5-F | 3880-3989 | CTATGCTGTGACGCATCCTG | Sense |
| CxFV-SD-5-R | 6232-6254 | AAGCCACAAATAGTCCATACAG | Reverse |
| CxFV-SD-6-F | 6048-6069 | ACTCAGTGCTGTTTCAAGG | Sense |
| CxFV-SD-6-R | 7204-7226 | CTCGTCCTGTTATCTCATCGTC | Reverse |
| CxFV-SD-7-F | 7021-7039 | GATTTCCTGGGAGACGAT | Sense |
| CxFV-SD-7-R | 8319-8337 | TCTCGCAACCTCTTCACG | Reverse |
| CxFV-SD-8-F | 8012-8033 | TTCTGTTGTAAGGTGCTGTCT | Sense |
| CxFV-SD-8-R | 9326-9344 | GGTTCTTCCCATTCGTCA | Reverse |
| CxFV-SD-9-F | 9140-9159 | CTGGACCAAGTGACTGACC | Sense |
| CxFV-SD-9-R | 10332-10350 | TGGCTGCGAGGGTGACTT | Reverse |
| CxFV-SD-10-F | 10016-10038 | AGTTTGATTGGTGAGCGGGACA | Sense |
| CxFV-SD-10-R | 10794-10815 | CCCGCAACAAGTCTCCTAACG | Reverse |
a The nucleotide position was according to the CxFV strain Tokyo genome.