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Detection limits and efficiencies of the multiplex and uniplex RT-QPCRs
| Virus | Detection limit (copy no. per μl) | Reaction efficiency | ||
|---|---|---|---|---|
| Multiplex | Uniplex | Multiplex | Uniplex | |
| BoHV-1 | 101 | 101 | 2.01 | 1.99 |
| BRSV | 101 | 101 | 2.14 | 2.00 |
| BPI3 i | 102 | 102 | 1.92 | 1.99 |
| BPI3 ii | 102 | 102 | 2.09 | 2.03 |
