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. 2012 Mar 26;10:8. doi: 10.1186/1478-811X-10-8

Figure 2.

Figure 2

Efficient knockdown of target genes in patient-derived childhood ALL-cells. A) ALL cells from sample ALL-53, ALL-50, ALL-168 and ALL-169 were transfected with siRNA against Caspase 8, p53 or NFκBp65 as in Figure 1C. Cells were harvested 48 h after transfection and Western Blot analysis of total cellular protein was performed. GAPDH served as loading control. Where indicated, xenograft samples did express only low levels of Caspase-8. B) ALL cells from sample ALL-53 (left panel) and ALL-54 (right panel) were transfected as in Figure 2A using two different siRNA sequences targeting Caspase-8. C) ALL cells of sample ALL-199 were repetitively xenografted within the identical passage (p6) and treated and analyzed as in Figure 2A. D) ALL cells of sample ALL-54 were xenografted in passage 3 (upper panel) and 4 (lower panel) and treated and analyzed as in Figure 2A. E). ALL cells from sample ALL-50 were simultaneously transfected and analyzed as in Figure 2A using siRNAs against NOXA and PUMA. F). ALL-199 (upper panel) and ALL-54 (lower panel) were transfected with siRNA against p53 as in Figure 2A and analyzed at 48 and 120 h for the stability of the knockdown.