Table 1.
Bacterial strains and plasmids used in this study
| Strain | Description | Reference |
|---|---|---|
| APEC O1 | O1:K1:H7; fyuA, sitA, chuA, irp2, iroN, ireA, tsh, iucD, fimC, iss, ompA, vat, traT; contains four plasmids, including pAPEC-O1-ColBM | [14] |
| BJ502 | E. coli K12, ΔtktA | [15] |
| DH5α | E. coli K12 | |
| APEC O1-Mtkt1 | APEC O1 derivative, Δtkt1 | this study |
| APEC O1-MtktA | APEC O1 derivative, ΔtktA | this study |
| S17λpir | recA thi pro hsdR- M+ RP4::2-Tc::Mu::Km Tn7 lysogenized with λpir phage | [12] |
| S17pGP tkt1 | S17λpir with plasmid pGP704 tkt1 | this study |
| APEC O1-Ctkt1 | APEC O1 Mtkt1 with plasmid pGP tkt1 inserted into bacterial chromosome | this study |
| APEC O1-P1 | APEC O1 Mtkt1 with plasmid pBAD tkt1 | this study |
| BJ502-P1 | BJ502 with plasmid pBAD24 | this study |
| BJ502-P2 | BJ502 with plasmid pBAD tkt1 | this study |
| BJ502-P3 | BJ502 with plasmid pBAD tktA | this study |
| APEC collection | 452 APEC strains isolated from lesions of birds clinically diagnosed with colibacillosis | [17] |
| Avian fecal E. coli | 106 avian fecal E. coli strains were isolated from the feces of apparently healthy birds | [17] |
| UPEC collection | 200 uropathogenic E. coli strains from from MeritCare Medical Center in Fargo, North Dakota | [18] |
| NMEC collection | 91 human neonatal meningitis-causing E. coli strains from the cerebrospinal fluid of newborns in the Netherlands, isolated from 1989 through 1997 and from Dr. K. S. Kim at John Hopkins. | [19] |
| Plasmids | ||
| pGP704 | Apr, suicide plasmid | [20] |
| pBAD24 | Apr, expression plasmid with arabinose-inducible promoter | [21] |
| pKD46 | Apr; expresses λ red recombinase | [22] |
| pKD3 | cat gene, template plasmid | [22] |
| pGP tkt1 | pGP704 derivative harboring tkt1 gene | this study |
| pBAD tkt1 | pBAD24 derivative, tkt1 gene under the control of PBAD | this study |
| pBAD tktA | pBAD24 derivative, tktA gene under the control of PBAD | this study |