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. 2012 Mar 31;31(1):31. doi: 10.1186/1756-9966-31-31

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Copyright ©2012 Zhu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Migration of human breast cancer cells in the presence of BLyS. 0.1 ng/ml, 1 ng/ml and 10 ng/ml BLyS were added in the lower chamber. 2% FBS and 1% FBS added in the lower chamber were used as positive chemoattractant and negative chemoattractant respectively. (A) MDA-MB-435. (B) MDA-MB-231. (C) MDA-MB-468. Average numbers of cells migrating in five randomly selected fields were counted after 8 h (original magnification 200 ×). Data are means of triplicate samples with ± SD; *, P < 0.05, **, P < 0.01, ***, P < 0.001, vs 1% FBS under normoxia. #, P < 0.05, ##, P < 0.01, ###, P < 0.001, vs 1% FBS under hypoxia.