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. 2012 Apr 5;11:20. doi: 10.1186/1476-4598-11-20

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Copyright ©2012 Schultz et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SPARC does not enhance or suppress radiation therapy (RT). A. Representative fluorescent images of control C1.1 GFP- and H2 SPARC-GFP-expressing clonogenic colonies (×40 magnification). B. Average colony forming efficiency ± SD of untreated C1.1 and H2 cells (left panel). Average surviving fraction ± SD of C1.1 and H2 cells exposed to 0 or 1-5 Gy (middle panel) or 0, 5 or 10 Gy (right panel), plating 3000 cells/60-mm dish. Plating 1000 cells/60-mm dish gave similar results. C. Average colony forming efficiency ± SD of untreated LN443 cells transfected with control (Csi) or SPARC siRNA (SPARCsi) (left panel). Average surviving fraction ± SD of LN443 cells transfected with Csi or SPARCsi exposed to 0, 5 or 10 Gy (right panel) plating 750 cells/60-mm dish. Plating 1500 cells/60-mm dish gave similar results.