Typical segmentation result for all five segmentation methods. Typical MaR segmentation shown in red for the automatic segmentation Segment MaR (panel A), second observer delineation (panel B), the threshold methods of two standard deviations from remote (2SD) (panel C), full width half maximum (FWHM) (panel D) and Otsu (panel E), compared to manual delineation by the reference observer, shown in yellow, all within the manual delineation of myocardial borders (shown in white). The same patient, short-axis slices, manual delineation of myocardial borders and manual reference delineation of MaR is used for all methods and shown from most basal slice in the left of the panel to the most apical slice in the right of the panel. Note the continuous appearance of the segmentation for Segment MaR and manual delineation by the reference and second observer compared to the threshold methods of 2SD, FWHM and Otsu.