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. 2012 May 10;7(5):e37132. doi: 10.1371/journal.pone.0037132

Figure 1. Recombinant NGR-hPK5 protein purification and identification.

Figure 1

A , Expressed and purified NGR-hPK5 samples were separated by Tricine-SDS-PAGE, M: molecular weight markers. The culture supernatant was collected and concentrated using ammonium sulfate precipitation (70%), dissolved in buffer A (lane 1). After the ion exchange purification by DEAE-Sepharose Fast Flow column, NGR-hPK5 was eluted with 0.1 M NaCl in buffer B (lane 2), whereas the bulk impurities were eluted with 0.5 M NaCl in buffer B (lane 3). B , Western blot analysis of hPK5 (lane 1) and NGR-hPK5 (lane 2) proteins using anti-human plasminogen antibody.