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. 2012 May 10;7(5):e35606. doi: 10.1371/journal.pone.0035606

Figure 2. CD55 is upregulated by poly(I:C) and IL-1β on synovial fibroblasts.

Figure 2

RA-derived synovial fibroblasts (A, C-F) and dermal fibroblasts (B) were starved overnight and subsequently stimulated for 2 days with 100 ng/ml TNFα, 100 ng/ml IFNγ, 100 ng/ml IL-1β, 1 ng/ml IL-6, 100 U/ml IFNα, 100 µg/ml LTA (TLR2 ligand), 100 µg/ml poly(I:C) (TLR3 ligand), 10 µg/ml LPS (TLR4 ligand), 100 µg/ml imiquimod (TLR7 ligand), or 10 µg/ml CpG oligonucleotides (TLR9 ligand). Expression of CD55 (A and B), CD46 (C) and CD59 (D) was studied by flow cytometry. E, Upregulation of CD55 in response to increasing concentrations of poly(I:C). F, Inhibition of CD55 upregulation by chloroquine (HCQ), an inhibitor of endosomal acidification, added prior to poly(I:C) stimulation. Indicated is the relative protein expression as percentage of the medium control (mean ± SD, n = 6 (A) and 3–5 (B-F)). *, p<0.05; **, p<0.005.