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. 2011 Oct 19;107(1):473–483. doi: 10.1152/jn.00741.2011

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Fig. 3. — 5-HT-induced inward current is a nonselective cation current mediated by transient receptor potential (TRP) channels. A: TTX (1 μM) blocks a tonic inward current at −55 mV, indicating that persistent Na⁺ current (I_NaP) is active at resting membrane potential (Vm). B: 5-HT (20 μM) induces an inward current in the presence of TTX (1 μM). C: pooled data show that TTX has no effect on 5-HT-induced inward current. D: 5-HT (20 μM) induces an inward current in the presence of ZD 7288 (ZD; 20 μM), a selective I_h channel blocker. E: superimposed traces showing the hyperpolarizing voltage step-activated inward current (I_h) in the absence (black) or the presence (red) of 5-HT (20 μM). F: pooled data show that 5-HT (20 μM) has no effect on I_h. G: population data showing the current-voltage relationship (I-V) of 5-HT-induced inward current (black) with a linear least-squares best fit (red) indicating reversal potential at −19.2 mV. H: repeated exposure to 5-HT (20 μM) induces identical inward currents (top trace). The inward current is abolished by 2-APB (100 μM), a broad-spectrum TRP channel blocker (middle trace). The inositol-1,4,5-trisphosphate (IP₃) signaling pathway inhibitor Xestospongin C (XeC; 5 μM) does not alter 5-HT-induced inward current. I: population bar graph showing the 5-HT-induced inward current is blocked by 2-APB (**P < 0.001) but not by XeC.