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Effects of recombinant FGF23. The changes of serum phosphate (A) and calcium (B) concentrations in mice treated with purified recombinant protein (n = 6; filled columns) or vehicle (n = 6; open columns). FGF23 was i.p. injected three times with intervals of 5 h. Blood samples were collected at 12 and 24 h after the first injection. Each column represents the mean ± SEM. *, P < 0.05; **, P < 0.001 by Student's t test.
