Figure 1. Decreased numbers and frequencies of DP thymocytes but equal numbers of SP thymocytes in Gαi2^−/− mice compared to control mice.

Flow cytometric analyses of the frequencies and total numbers of CD4⁺8⁺ (DP) (A–B), CD4⁺8⁻ (CD4 SP) (C–D) and CD4⁻8⁺ (CD8 SP) (E–F) thymocytes. Results from wt mice (n = 28–31) and Gαi2^−/− mice (n = 6–14) are shown as frequencies or numbers of thymocytes from individual mice with the mean within each group presented as a horizontal line. The control group consisted of 4–9 weeks old wt mice whereas the Gαi2^−/− mice were 4–8 weeks old, grouped based on their colitis score; no and mild colitis (rectangles and triangles, respectively), moderate (dark gray circles) and severe colitis (black circles). Statistical analysis was performed using the Mann-Whitney non-parametric test and values of p≤0.05 were considered significant, *p≤0.05; **p≤0.01 and ***p≤0.001 between Gαi2^−/− mice and control mice or between Gαi2^−/− mice with different colitis scores.