Figure 5. PBs and Pab 1801-positive puncta simultaneously dissolve upon polysome stabilization.

U2OS cells were treated with CHX or PURO during one hour, and immunostained with the indicated antibodies. The Pab 1801 punctate signal vanished together with the PBs upon cycloheximide treatment. Both foci remained intact upon exposure to puromycin. The percentage of cells with PBs simultaneous stained with Dcp1a and Pab 1801 was evaluated in 100 cells from duplicate experiments for each treatment. The Pab 1801 foci were never observed free of Dcp1a, and PBs were always stained with the Pab 1801. Bar, 10 µm.