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. 2012 May 10;7(5):e36447. doi: 10.1371/journal.pone.0036447

Figure 9. Different procedures give the same immunostaining pattern of Pab 1801.

Figure 9

U2OS cells were treated as indicated and immunostained with Pab 1801 and Hedls. A, U2OS cells were fixed as usual in 4% PFA. B, live U2OS cells were treated with methanol-acetone during 3 minutes at room temperature (mild non-aq). C, live U2OS cells were treated with methanol-acetone during 20 minutes at −20°C (strong non-aq). D, live U2OS cells were permeabilized with 0.1% Triton X-100 for 10 minutes prior to fixation in PFA 4%. Nuclear staining with Pab 1801 looks stronger than in control cells. None of these treatments affected the cross-reaction of the Pab 1801 with PBs. Bars, whole cells, 10 µm; magnifications, 1 µm.