Figure 11. The Pab 1801 does not recognize Drosophila PBs, and stains a subset of PBs in rat neurons.

A, S2R+ cells were immunostained with the Pab 1801 and an antibody against Ge-1/Hedls. No signal of the Pab 1801 was observed. Bars, 10 µm. B, rat primary neurons were simultaneously stained with Pab 1801, Hedls and Tubulin β III. Pab 1801 puncta and Hedls colocalize partially. Bars, whole neurons, 10 µm; dendrites, 1 µm. C, representative dendritic fragments showing immunostaining against Pab 1801 and the PB components Xrn1, Dcp1a and Rck/p54. As above, Pab DO1 yielded no signal. Bars, 1 µm.