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. 2012 May 10;7(5):e36553. doi: 10.1371/journal.pone.0036553

Figure 2. Validation of candidate sRNAs expressed from unique nstSGR in either PAO1 or PA14.

Figure 2

A selection of nstSGRs listed in Table S2, unique in either PAO1 or PA14, were inspected by Northern blot for the expression of sRNAs. Total RNA was extracted from both PAO1 (○) and PA14 (•) grown in the same conditions as for sRNA-Seq. Equal amounts of RNA (8 µg) from both strains were blotted and probed with radiolabelled riboprobes (0002 and 0021) or oligos (Table S1) complementary to nstSGR regions with the highest read coverage, as detailed in Materials and Methods. Validated unique sRNAs in PAO1 or PA14 are shown in (A) and (B), respectively. For SPA0014, 0015, 0018, 0019, signals detected in both strains (dots on the left of PAO1 lanes) can be due to aspecific probe hybridization. The ladder of molecular weight markers is shared by (A) and (B). (nt): nucleotides.