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. 2012 May 10;7(5):e36501. doi: 10.1371/journal.pone.0036501

Figure 1. Enhancer screen of evolutionary conserved sequences within TCF7L2 association interval.

Figure 1

(A) The TCF7L2 gene is shown above with the 92-kb T2D-associated internal highlighted in red. A red asterisk marks SNP rs7903146. Sequence conservation between human-opossum is given (ECR genome browser, [43]). The 13 evolutionary conserved regions (ECRs) harboring conservation down to opossum are highlighted in grey and numbered below. Regions exhibiting reproducible enhancer activity are marked in red. (B) Reproducible expression profiles from ECRs at embryonic day 15.5 (E15.5) are shown. Expression can be seen in the stomach (ECR 1), neurons near the spine (ECR 3), limb bones and axial skeletion (ECR 4), forebrain (dorsal view, ECR 5), walking pads (ECR 6), limb vasculature (ECR 8), brain vasculature (dorsal view, ECR 9), limbs and midbrain (dorsal view, ECR 11), and phalangies (ECR 13). In ECRs 4, 6, 8, 11 and 13, both forelimb (left) and hindlimb (right) images are given. Reproducible regulatory activities were not identified for ECRs 2, 7, 10 and 12.