Figure 3. HRB1 interacts with PP7 in vivo.

(A) Accumulation of HRB1∶Myc and PP7∶Myc as driven by the 35S CaMV promoter in the leaves of 4-week-old transgenic plants in the dark and in response to 4.93 µmol/m²/s blue light for 1, 3, 6 and 9 hours. The blots were stripped and re-probed with an antibody against ß-tubulin. (B) His affinity-precipitation of PP7∶Myc with GFP∶His or HRB1∶GFP∶His in the dark and under 4.93 µmol/m²/s blue light for 2 hours in Nicotiana leaves. (C) Co-localization of HRB1∶CFP and PP7∶YFP driven by the 35S CaMV promoter in Nicotiana leaf epidermal cells (upper panel) or driven by their native promoters in 2-week-old Arabidopsis leaf guard cells (lower panel) under 4.93 µmol/m²/s blue light for 2 hours. Propidium iodide (PI) fluorescence is shown in pseudo color to illustrate the cell shape.